Introduction
In recent years, the detection and identification of nucleic acids using point-of-care total nucleic acid analysis systems are in demand in various clinical and industrial settings. Many system-based platforms including isothermal amplification using loop-mediated isothermal amplification technology have helped immensely to achieve enhanced nucleic acid amplification by increasing surface area to volume ratio.
As polymerase chain reaction (PCR) is a widely used and expensive DNA amplification method as it involves multiple thermo-cycling steps which uses expensive thermo-cyclers. Isothermal amplification of nucleic acids using loop-mediated isothermal amplification eliminates such thermo-cycling steps, thereby reducing test cost and increasing assay efficiency and quality.
Besides, loop-mediated isothermal amplification is a nucleic acid based point-of-care diagnostic platforms that require fingerpick volumes of cell lysis, nucleic acid extraction, and purification reagents prior to amplification.
Significant Improvements in Amplicon Detection is Key for Market of Loop-Mediated Isothermal Amplification
Over the past decade, there has been significant improvements in monitoring loop-mediated isothermal amplification Amplicon detection during end-point assay reaction and also during real-time analysis. Optical, electrical, electrochemical, and pH-sensing mechanisms are used for real-time monitoring of loop-mediated isothermal amplification Amplicon. With advancements in amplification technology, loop-mediated isothermal amplification techniques have become promising alternatives to the traditional gold standard, i.e., PCR.
Besides loop-mediated isothermal amplification seems to be a reliable and robust isothermal amplification technique for detection of pathogens in patient samples. With number of research publications using loop-mediated isothermal amplification method increasing, its popularity can be noticed. Since 2001 there has been exponential growth in the number of publications and citations related to the applications of loop-mediated isothermal amplification in various filed such as in foo bio-medicine; environmental science; and food and beverage industries.
Provision for High Throughput and Complex Analysis of Loop-Mediated Isothermal Amplification
Since its inception in 2001, several loop-mediated isothermal amplification based assays have been developed over the years for simultaneous detection of pathogens in biological samples and food products. With revolutionary microfluidic technology, provision for high throughput and complex analysis of samples could be achieved using loop-mediated isothermal amplification.
Advance fluidic components such as reaction chambers, micro-valves, micro-pumps, and micro-dispensers can be integrated in Lab-on-chip-based loop-mediated isothermal amplification assays to provide both micro as well as Nano droplet-size samples for multiplexing and high-throughput analysis.
A novel microfluidic platform called SlipChi (loop-mediated isothermal amplification) with the ability of high throughput analysis of Nano liter sized biological samples have been commercialized. The platform allows multitasking and doesn’t require need for valves and on-chip based micro pumps for handling for samples. It also reduces the amount of samples required in Pico and Nano liter volumes.
A loop-mediated isothermal amplification based digital microfluidic device was also developed for detecting amplified nucleic acids. Using this loop-mediated isothermal amplification system, a throughput of 1 million droplets in 110 min could be obtained with limit of detection (LOD) of 600 copies/min.
Distribution & Collaboration Agreements to Increase Loop-Mediated Isothermal Amplification Product Reach
Key players in the global loop-mediated isothermal amplification market are adopting distribution & licensing agreements with regional players from MEA, East and South Asia as an expansion strategy in the regions.
Players of loop-mediated isothermal amplification market are looking to form collaborations to improve the sale of DNA polymerase and other loop-mediated isothermal amplification based reagents in all regions and hence, gain maximum revenue share in the market.
Manufacturers of loop-mediated isothermal amplification products have developed several products that have the ability to be used in both research applications as well as for clinical diagnostics.
Loop-Mediated Isothermal Amplification Market: Competitive Landscape
Key manufacturers of instruments and reagents used in loop-mediated isothermal amplification procedures are focusing on differentiating strategies to increase not only their product portfolio but also their regional dominance. Most of the companies operating in a loop-mediated isothermal amplification are focused on aggressive pricing strategies in order to lead the market for loop-mediated isothermal amplification.
Some other key market strategies followed by loop-mediated isothermal amplification companies include research and development agreements to increase product development, adopting distribution & licensing agreements with regional players, and establishment of network of distribution and marketing agreements to ensure availability of products to different end use segments globally.
Loop-Mediated Isothermal Amplification Market: Additional Questions Answered
- What is the revenue potential of the Loop-Mediated Isothermal Amplification Markets across North America and Europe?
- Who are the key competitors and what are their portfolios in the Loop-Mediated Isothermal Amplification Market?
- What are the major challenges influencing the growth of the Loop-Mediated Isothermal Amplification Market?
- How is intervention from regulatory authorities shaping the growth of the Loop-Mediated Isothermal Amplification Market?
Research Methodology
The Loop-Mediated Isothermal Amplification Market has been estimated based on installed-based approach to obtain market value (US$) and volume (units) of instruments and company share analysis of key competitors to find consumption and market value of kits and reagents. The market was studied by estimating the base year market side in different region/countries.
The market was estimated based on product installed/consumed in the top 20 countries, globally. Other qualitative factors analyzed during market value estimation include awareness level of loop-mediated isothermal amplification procedure, product usage, and regional players. This information was further validated with rigorous primary research (including interviews, surveys, in-person interactions, and viewpoints of seasoned analysts) and secondary research (including verified paid sources, authentic trade journals, and resourceful databases).
The research study on the loop-mediated isothermal amplification market also includes top trends and macro- as well as micro-economic factors shaping the loop-mediated isothermal amplification market. With this approach, the report on the loop-mediated isothermal amplification market anticipates the industry attractiveness of every major segment in the loop-mediated isothermal amplification market over the forecast period.
Key Segment
Based on product type
- instruments
- incubation systemsTurbidimeters
- Agarose gel electrophoresis
- fluorescence measuring systems
- kits & reagents
- DNA polymerase
- Primer Mix
- DNA Polymerase &Primer mix
- dyes
- other reagents
Based on Technology
- microfluidic technology
- advanced infrared optical technology
- modern microsystem technology
Based on Application
- diagnostic
- research purpose
Based on End User
- hospital & laboratories
- research & academic institutes
- others
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by - Sudip Saha, Managing Director and Co-Founder of Future Market Insights
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