A blotting technique in molecular biology is defined as transfer of electrophoresed samples of DNA, RNA and proteins onto a suitable carrier such as nylon or nitrocellulose membrane and polyvinylidene difluoride (PVDF) membrane and subsequent fragment detection by probe hybridization. Southern blotting is a molecular biology technique used to detect specific DNA sequence in DNA samples.
The technique is named after its inventor Edward Southern. Southern blotting technique combines transfer of electrophoresis separated DNA fragments to a filter membrane (nitrocellulose or nylon) and subsequent fragment detection by probe hybridization. Similar techniques involving separation of macromolecules and probing for certain sequences or motifs have now been developed to study RNA and protein named as northern blotting and western blotting techniques respectively.
Southern blotting techniques is used in many areas such as in gene mapping, forensic and diagnostics studies. In regards to disease diagnosis the technique is used in diagnosis of congenital inherited disease such as congenital adrenal hyperplasia (CAH). In Southern blotting, DNA fragments that have been separated by gel electrophoresis from the intact gel is transferred onto a piece of nitrocellulose or nylon membrane.
The membrane is then exposed to a hybridization probe-a single DNA fragment with a specific sequence whose presence in the target DNA (or RNA) is to be determined. The probe DNA is labeled so that it can be detected by radiography, fluorescence, or chemiluminescence. The demand for global southern blotting market is rising tremendously in medical, forensic, agricultural fields and drug discovery.
In medical field, southern blotting technique is used for prognosis of cancer and in prenatal diagnosis of genetic diseases. Southern blotting technique also finds its application in diagnosis of HIV-1. In forensic science, southern blotting technique is used in sex determination and paternity testing.
The global southern blotting market is expected to rise during the forecast period due to increasing number of healthcare disorders affecting the large pool of population coupled with technological advancement and increasing adoption of southern blotting technique in various biotechnology and pharmaceutical industries.
The global market for southern blotting is expected to have a robust growth during the forecast period (2015-2025).
Factors contributing to the growth of global southern blotting market includes, increasing usage of southern blotting technique in the field of genetics, and growing demand for advancements and innovation in drug discovery and development. Other factors contributing towards the growth of global southern blotting marketis rising incidence of various diseases such as congenital adrenal hyperplasia and HIV-1 and demand for faster diagnosis.
In addition, increasing use southern blotting procedures in medical diagnostics, application in forensic science, are the key factors responsible for driving global southern blotting market.
However,factors such as, non-availability of device in developing countries along with emergence of alternative technologies, inadequate funding in developing countries, high pricing of products are expected to hamper overall market growth for global southern blotting market.
With rising incidence of diseases, rapid technological advancements in medical field, and increased spending in research and development, the global southern blotting market is expected to have a robust growth during the forecast period (2015-2025).
The global market for southern blotting is expected to register a healthy CAGR during the forecast period. Depending on geographic regions, global southern blotting market is segmented into seven key regions: North America, South America, Eastern Europe, Western Europe, Asia Pacific excluding Japan, Japan and Middle East & Africa.
North America holds the largest share in the global southern blotting market due to dramatic rise in HIV-1 prevalence, increasing production of genetically modified crops, rising government funding, and increase spending in research and development procedures. Europe holds the second position in terms of southern blotting market owing to adoption of advanced technology in biotechnology and research sector in this region.
Asia is expected to experience high growth rates for southern blotting market in next few years due to increasing incidences of HIV infections, growing biotechnological and pharmaceutical industries in this region, huge pool of aging population with different disease indications and increasing expenditure by several government on healthcare.
The significant development of the emerging economies of India and China will also lead to growth of this region.
Some of the key market players in global southern blotting market are Agilent Technologies, ThermoFisher Scientific, Perkin Elmer Inc., Applied Bio systems, Aplegan, BioRad Laboratories, Cleaver Scinetific Ltd, Pall Life Science Laboratories.
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Global Southern Blotting Marketis segmented on the basis of product, technology, application, end use and geography as following:
By Product
By Application
By End User
Southern Blotting Market
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